Journal:

Article Title: Antibody blockade of the Cripto CFC domain suppresses tumor cell growth in vivo

doi: 10.1172/JCI200317788

Figure Lengend Snippet: (a) Diagram of mature hCr protein: N-terminal tip (black), N-terminal region (blue), EGF-like domain (red), and CFC domain (green). Amino acid 169 is N-terminal to the GPI-linkage site. Asterisks indicate positions of mutants described in Table ​Table1.1. (b) Western blot of Cripto expressed in CHO cells (recombinant) or endogenously expressed in NCCIT and GEO tumor cell lines using A10.B2.18. (c) Immunofluorescent staining of cell surface Cripto on human breast tumor tissue with A10.B2.18 (left) or control antibody 1E6 (right). (d) Immunohistochemical staining of tumor sections with anti-Cripto mAb’s A10.B2.18 (breast and NCCIT) and B3.F6.17 (colon and GEO) or mouse IgG as a negative control. Color variations result from the use of different substrates, either Vector NovaRED or DAB substrate kits (Vector Laboratories Inc.).

Article Snippet: Color was developed using Vector NovaRED or DAB substrate kits (Vector Laboratories Inc.).

Techniques: Western Blot, Recombinant, Staining, Immunohistochemical staining, Negative Control, Plasmid Preparation